Homework Answers
In cation exchange resin, positively charged Amino acid bind to resin and elute last. Since Arg contain basic Amino group which exist as positively charged hence bind to resin and elute last. Asp contain acidic group which exist as negatively charged hence elute first. So, order of elution is :
(Last) Arg < Ser < Asp (first)
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Can you clearly explain the order of elution and exactly WHY
they elute in that specific...
Question 5.A mixture contains Gu, His, Pro, Lys and need to be separated using Dowex 50...
Question 5.A mixture contains Gu, His, Pro, Lys and need to be separated using Dowex 50 cation exchange, prepared in a loading buffer at pH 2.0 : the following buffers are available :- -Amino acid mixture in 0.04 M citrate buffer, at pH 2.0. -0.04 M citrate buffer at 4.5 -0.04 M citrate buffer at 8.0 -0.04 M citrate buffer at 10.5 Assume these amino acids elute only at pH above their perspective pl. Support your answers with clear explanations....
need only part a and C. please and thank you 6. Suppose you have a mixture...
need only part a and C. please and thank you
6. Suppose you have a mixture of five proteins listed in the table below: Protein pl Mol. Welght, kDa Homer 4.6 69 Liza 10.7 13 Marge Maggie Bart 5 40 6.4 8.5 7 27 Indicate the order in which the proteins elute from a Bio-Gel p-10 gel-filtration column (starting with the on that elutes first). Is this the best resin to separate all the components of this mixture? Why? If...
#2 is Q HiTrap column where it is anion exchange column not cation 2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrin...
#2 is Q HiTrap column where it is anion exchange column not
cation
2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
The order of amino acids (Lys, Asp, ILE) eluted from an anion exchange column at PH 7 should be: KDI DIK DKI IKD no correct answer Please explain why the answer is no correct answer. Thank you.
The order of amino acids (Lys, Asp, ILE) eluted from an anion exchange column at PH 7 should be: KDI DIK DKI IKD no correct answer Please explain why the answer is no correct answer. Thank you.
Q1: You have two short peptides: Peptide A) KVDFSA; Peptide B) IRGTKL a. Write the chemical...
Q1: You have two short peptides: Peptide A) KVDFSA; Peptide B) IRGTKL a. Write the chemical structure of peptide A as it would appear at pH 7. Include all charges and begin from the N-terminus b. determine the net charge on each of the peptides at pH 2, 7, and 11 c. You would now like to separate the two peptides. You decide to use a cation exchange column at pH 7 A cation exchange column is composed of negatively...
answer for question b c,d and e only please. a) b) c) Explain why the affinity...
answer for question b c,d and e
only please.
a) b) c) Explain why the affinity of alkali metal ions for a cation exchange resin increases from Lit to Nat to K. [6 marks] Explain the physical basis for the separation of molecules in size exclusion chromatography. [6 marks) When a mixture of ethanol, n-propanol, n-butanol and n-pentanol (see Figure 5) is analysed by capillary gas-liquid chromatography, what order would you expect the compounds to elute? [2 marks] Explain your...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
Name Standard Reduction Potentials of Some Biologically Important Half-Reactions TABLE 13-7 Amino...
Name Standard Reduction Potentials of Some Biologically Important Half-Reactions TABLE 13-7 Amino acids pki k2 pkR Half-reaction 2.34 9.60 2.34 9.69 1.99 10.96 2.32 9.62 2.36 9.60 2.36 9.68 Ubiquinone + 2H+ + 2e-→ ubiquinol + H: 0.045 Fumarate:-+ 2H. + 2e-→ succinate"- 2H+ + 2e-→ H2 (at standard conditions, pH 0) 0.000 Crotonyl-CoA+2H +2butyryl-CoA0.015 Oxaloacetate:-+ 2H+ + 2e-→ malate"--0.166 Pyruvate +2H2elactate Acetaldehyde + 2H+ + 2e-→ ethanol FAD +2HFADH2 Glutathione + 2H+ + 2e- Alanine Proline 0.031 228 921...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads,...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads, (a) 1.0M NaCI was used, (b) HRP was used, (c) the sephadex beads were washed twice with NaCl, (d) glucose was used, (e) the elution buffer contained a carbohydrate binding protein. 2. A specific biological property is used to separates macromolecules in (a) affinity chromatography (b) TLC, (c) ion exchange chromatography, (d) electrophoresis, (e) protein assay 3. In order to separate proteins from lectins...
Biochem help 2 14. What would be the net charge on the dipeptide Ser-His at pH-...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
Question 5.A mixture contains Gu, His, Pro, Lys and need to be separated using Dowex 50 cation exchange, prepared in a loading buffer at pH 2.0 : the following buffers are available :- -Amino acid mixture in 0.04 M citrate buffer, at pH 2.0. -0.04 M citrate buffer at 4.5 -0.04 M citrate buffer at 8.0 -0.04 M citrate buffer at 10.5 Assume these amino acids elute only at pH above their perspective pl. Support your answers with clear explanations....
need only part a and C. please and thank you
6. Suppose you have a mixture of five proteins listed in the table below: Protein pl Mol. Welght, kDa Homer 4.6 69 Liza 10.7 13 Marge Maggie Bart 5 40 6.4 8.5 7 27 Indicate the order in which the proteins elute from a Bio-Gel p-10 gel-filtration column (starting with the on that elutes first). Is this the best resin to separate all the components of this mixture? Why? If...
#2 is Q HiTrap column where it is anion exchange column not
cation
2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
Q1: You have two short peptides: Peptide A) KVDFSA; Peptide B) IRGTKL a. Write the chemical structure of peptide A as it would appear at pH 7. Include all charges and begin from the N-terminus b. determine the net charge on each of the peptides at pH 2, 7, and 11 c. You would now like to separate the two peptides. You decide to use a cation exchange column at pH 7 A cation exchange column is composed of negatively...
answer for question b c,d and e
only please.
a) b) c) Explain why the affinity of alkali metal ions for a cation exchange resin increases from Lit to Nat to K. [6 marks] Explain the physical basis for the separation of molecules in size exclusion chromatography. [6 marks) When a mixture of ethanol, n-propanol, n-butanol and n-pentanol (see Figure 5) is analysed by capillary gas-liquid chromatography, what order would you expect the compounds to elute? [2 marks] Explain your...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
Name Standard Reduction Potentials of Some Biologically Important Half-Reactions TABLE 13-7 Amino acids pki k2 pkR Half-reaction 2.34 9.60 2.34 9.69 1.99 10.96 2.32 9.62 2.36 9.60 2.36 9.68 Ubiquinone + 2H+ + 2e-→ ubiquinol + H: 0.045 Fumarate:-+ 2H. + 2e-→ succinate"- 2H+ + 2e-→ H2 (at standard conditions, pH 0) 0.000 Crotonyl-CoA+2H +2butyryl-CoA0.015 Oxaloacetate:-+ 2H+ + 2e-→ malate"--0.166 Pyruvate +2H2elactate Acetaldehyde + 2H+ + 2e-→ ethanol FAD +2HFADH2 Glutathione + 2H+ + 2e- Alanine Proline 0.031 228 921...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads, (a) 1.0M NaCI was used, (b) HRP was used, (c) the sephadex beads were washed twice with NaCl, (d) glucose was used, (e) the elution buffer contained a carbohydrate binding protein. 2. A specific biological property is used to separates macromolecules in (a) affinity chromatography (b) TLC, (c) ion exchange chromatography, (d) electrophoresis, (e) protein assay 3. In order to separate proteins from lectins...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
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