Question

Name Standard Reduction Potentials of Some Biologically Important Half-Reactions TABLE 13-7 Amino acids pki k2 pkR Half-reaction 2.34 9.60 2.34 9.69 1.99 10.96 2.32 9.62 2.36 9.60 2.36 9.68 Ubiquinone + 2H+ + 2e-→ ubiquinol + H: 0.045 Fumarate:-+ 2H. + 2e-→ succinate"- 2H+ + 2e-→ H2 (at standard conditions, pH 0) 0.000 Crotonyl-CoA+2H +2butyryl-CoA0.015 Oxaloacetate:-+ 2H+ + 2e-→ malate"--0.166 Pyruvate +2H2elactate Acetaldehyde + 2H+ + 2e-→ ethanol FAD +2HFADH2 Glutathione + 2H+ + 2e- Alanine Proline 0.031 228 921 1.83 9.13 2.20 9.11 10.07 2.38 9.39 -0.185 -0.197 -0.219* Methionine Tryptophan 2 reduced glutathione 2.21 9.15 2.11 9.62 1.96 10.28 8.18 2.02 8.80 1s + 2H+ + 2e-→H,s Lipoic acid + 2H +2edihydrolipoic acid -029 Cysteine Asparagine Glutamine Lysine Histidine Arginine NAD+ + H+ + 2e-→ NADH NADP+ + H+ + 2e-→ NADPH Acetoacetate + 2H+ + 2e- → -0.320 9.13 2.18 895 10.53 1.82 9.176.00 2.17 9.04 12.48 -0.346 B-hydroxybutyrate α-Ketoglutarate + CO2 + 2H + + 2e- isocitrate 1.88 9,60 365HHa (at pH 7) 2.19 9.674.25 3.65 -0.414 2H. + 2e-→ H2 (at pH 7) Ferredoxin (Fe3+) + e- → ferredoxin (Fe2+) -0.432 27.(a) Calculate the net charges on the following peptides at pH-7.0? Show steps. (7.5 points) Peptide 1: Ala-Glu-Gly-Phe-Asp Peptide 2: Ala-Glu-Ser-Phe-Arg Peptide 3: Ala-Val-Gly-Asp-Phe (b) Based on the net charges of the 3 peptides, what would be the order of elution of the peptides from a cation exchange column? Explain your reasoning? (4.5 points)

Answer 1