Question

For each of the following enzyme regulation mechanisms/situations below, list whether it will:

A. Always decrease activity V

B. Always increase activity V

C. May increase or decrease activity V

D. May decrease or possibly have minimal effect on activity V

E. Will definitely have no significant effect on activity V

1. Competitive Inhibitor with [I] > Ki and [S] < Km

2. Noncompetitive inhibitor with [I] > Ki and [S] < Km

3. Phosphorylation of Enzyme

4. Product Inhibition with [P] > Ki and [S] < Km

5. Pseudosubstrate sequence is present on the enzyme and [S] < Km

6. 150% availability of needed cofactor

7. 50% of needed cofactor is gone

8. Uncompetitive inhibitor with [I] > Ki and [S] < Km

9. Increase substrate when [S] < Km

10. Increase substrate when [S] >> Km

Enter your answer as 9 letters as per ABCDEABC according the 9 items above.

Answer 1

1) E (in competitive inhibition, activity remains same but km is affected)

2) A (in noncompetitive inhibition, activity is increased but km remains same)

3) C (phosphorylation may increase or decrease activity of the enzyme)

4) A (in product inhibition, the activity starts decreasing)

5)

6) E (extra cofactor does not have any role in kinetics)

7) D (less than needed cofactor may decrease kinetics)

8) A (uncompetitive inhibitors decrease activity as well as km)

9) B (increasing substrate concentration always increase enzyme activity upto saturation point)

10) A (when S>>km then substrate inhibition occurs as a result rate of product formation decreases)

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