Question

Question #4 (5 pts): Select a restriction enzyme, determine its cleavage sites, which organism is was derived from and how this enzyme doesn't cleave in the organism it is derived (minus points if you choose enzymes found in the text)?

Answer 1

Restriction enzyme - EcoRI ( pronounced 'eco R one' ) EcoRI is isolated from species E.coli . The Eco part of the enzyme's name originates from species from which it was isolated , while the R represents the particular strain,in this case RY13. The last part of its name , the I , denotes that it was the first enzyme isolated from this strain.

Restriction enzymes are part of restriction modified system.They doesnot cleave in the organism it is derived becuse that is methylated so that it protects it from restriction enzymes.

EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT . other restriction enzymes, depending on their cut sites,can aiso leave 3' overhangs or blunt ends with no overhangs.