Question

Question 2 4 pts Which characteristics are useful in a barcoding marker for identifying taxonomic diversity? (2 answers) O Has regions that are highly variable among species, but are conserved within species Is conserved throughout the locus so that primers will work anywhere in the locus Mutates so quickly that every sequence is unique, even very different among individuals from the same species O Is flanked by highly conserved regions where primers will reliably bind to the template DNA.

Answer 1

• DNA biocode markers are short DNA sequences, which have unique characteristics for identification of a species.
• Thus, information about a species may be obtained and its taxonomical or ecological position may be determined by sequencing the biocode marker.
• A barcode locus is needed to have lower intraspecific and higher interspecific divergence, referred to as bar code gap.
• The highly variable regions among regions help in distinguishing species.
• The conserved regions may be used for identification of the specific species.
• The marker should be flanked with highly conserved region.
• This facilitates binding of universal primer and help in amplification.

Answer: option 1 and 4