The single-point standard addition method was used in the determination of phosphate by the molybdenum blue method. A 2.00-mL urine specimen was treated with molybdenum blue reagents to produce a species absorbing at 820 nm, after which the sample was diluted to 100 mL. A 25.00-mL aliquot of this solution gave an absorbance of 0.428 (solution 1). Addition of 1.00 mL of a solution containing 0.0500 mg of phosphate to a second 25.0-mL aliquot gave an absorbance of 0.517 (solution 2). Use these data to calculate the mass of phosphate in milligrams per millimeter of the specimen.
The single-point standard addition method was used in the determination of phosphate by the molybdenum blue method. A 2.00-mL urine specimen was treated with molybdenum blue reagents to produce a spec...
The single-point standard addition method was used in the determination of phosphate by the molybdenum blue method. A 2.00-mL urine specimen was treated with molybdenum blue reagents to produce a species absorbing at 820 nm, after which the sample was diluted to 100 mL. A 25.00-mL aliquot of this solution gave an absorbance of 0.428 (solution 1). Addition of 1.00 mL of a solution containing 0.0500 mg of phosphate to a second 25.0-mL aliquot gave an absorbance of 0.517 (solution...
A 2.00-mL urine sample was treated with molybdenum blue reagent to produce a species absorbing at 820nm, after which the sample was diluted to 100mL. A 25.00mL aliquot of this solution gave an absorbance of 0.428. Addition of 1.00 mL of a standard solution containing 0.0500 mg/mL of phosphate to a second 25.00-mL aliquot gave an absorbance of 0.517. Use these data to calculate the concentration of phosphate in milligrams per milliliter of the original urine sample.
The urine sample containing phosphate was treated with molybdenum blue reagent to produce a species absorbing at 820 nm with an absorbance of 0.428. Then 1.00 mL of a 5.00 g/L phosphate standard was added to 104.0 mL of the urine sample. The spiked urine sample gave an absorbance of 0.517. Find the original concentration of phosphate in g/L in the urine sample. Correct sigfigs!