5. (12pts) You have discovered a novel cell-signaling pathway, in which cells stimulated with a hormone, Simpson, leads to a cell death response. The pathway involves 5 proteins with the following si...
5. (12pts) You have discovered a novel cell-signaling pathway, in which cells stimulated with a hormone, Simpson, leads to a cell death response. The pathway involves 5 proteins with the following signaling: Simpson (signal) Homer --I Marge > Bart Lisa MaggieCell growth (response) A. Do you expect the Simpson stimulus to lead to increased or decreased levels of cell growth? Please provide your reasoning. B. You construct a series of cell lines with mutations in each one of these genes that are always active (ex. When expressed, Maggie* is always active, regardless of other proteins.) In the table below, please predict (1) if there will be high or low levels of cell growth, (2) predict if the level of cell growth is sensitive to stimulus by Simpsorn. Mutant Cell growth? Homer Marge* Bart Lisa Maggie Sensitive to Simpson? (Yes or no) (High or low) C. Suppose you construct a cell line with both the Lisa and Maggie* gain-of-function mutations. Please predict if there will be high or low levels of cell growth, and provide your reasoning. D. Suppose you determine Marge is a GEF for Bart. Please propose a mutation in Bart that would make it insensitive to whether Marge is active or not.
5. (12pts) You have discovered a novel cell-signaling pathway, in which cells stimulated with a hormone, Simpson, leads to a cell death response. The pathway involves 5 proteins with the following signaling: Simpson (signal) Homer --I Marge > Bart Lisa MaggieCell growth (response) A. Do you expect the Simpson stimulus to lead to increased or decreased levels of cell growth? Please provide your reasoning. B. You construct a series of cell lines with mutations in each one of these genes that are always active (ex. When expressed, Maggie* is always active, regardless of other proteins.) In the table below, please predict (1) if there will be high or low levels of cell growth, (2) predict if the level of cell growth is sensitive to stimulus by Simpsorn. Mutant Cell growth? Homer Marge* Bart Lisa Maggie Sensitive to Simpson? (Yes or no) (High or low) C. Suppose you construct a cell line with both the Lisa and Maggie* gain-of-function mutations. Please predict if there will be high or low levels of cell growth, and provide your reasoning. D. Suppose you determine Marge is a GEF for Bart. Please propose a mutation in Bart that would make it insensitive to whether Marge is active or not.