Question

Which of the following sentences is nottrue about alcohol dehydrogenase a. The enzyme uses NADH as cofactor. b. The enzyme transfers the hydride, H- between the substrates. c. If yeast alcohol dehydrogenase is reacted with CH3CD2OH, the generated NADH will contain deuterium in the pro-R position. d. If deuterium NAD+ is reacted with CH3CH2OH, the generated aldehyde will contain deuterium. e. If a pro-R deuterium NADH is reacted with Glyceraldehyde-3-phosphate dehydrogenase, deuterium will be remained in NAD+

Answer 1

The statements not true about alcohol dehydrogenase are a and d

a. The enzyme uses NADH as cofactor.

The enzyme uses NAD+ as cofactor not NADH as H+ transferred into NAD+ to form NADH

d) If deuterium NAD+ is treated with CH3CH2OH, the generated aldehyde will contain deuterium.

The statement is false as aldehyde forms by loosing hydrogen so if deuterium NAD+ reacted with alcohol there will not be any deuterium in aldehyde.