Question

Please answer it ALL completely and write clearly, thank you.

                          ( 10 marks, pls write briefly thank you)

                         ( 10 marks, PLS write briefly, Thank you)

High performance liquid chromatography (HPLC) deals with liquid samples at high pressures whereas mass spectrometer (MS) operates at normal temperatures and requires high vacuum conditions. Describe in detail hovw these very conflicting conditions are taken care of in the process of combining the two techniques resulting in a hybrid technique known as HPLC-MS. Use appropriate diagrams if needed to clarify your explanation. 4. (a) (b) Separation of the compounds 2,3,7,8-tetrachloro-dibenzo-p-dioxin and numerous other dioxin isomers are usually done using capillary gas chromatography due to the required high efficiency of separation. In addition, 2,3-dichloro-7,8-dibromo and 2,3,7,8-tetrabromo isomers are sometimes found in the sample, making the separation and identification more difficult. How can gas chromatography-mass spectrometry (GC-MS) technique be used to distinguish the dioxins from the 2,3-dichloro-7,8-dibromo and 2,3,7,8- tetrabromo isomers?

Answer 1

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