You have been hired to study a new antibiotic produced by streptomyces colicolor. The drug has isolated after isolation and identification of streptomyces colicolor using a device known as the chip. your advisor wants to find out the mechanism of action of the drug and determine whether it has bactericidal or bacteriostatic effects.
a) What experiment could you do to determine the cellular target of the drug. briefly discuss two experiments
b) After many months of experiments and hard work, you have identified the gene cluster encoding the enzymes necessary for the production of this antibiotic. describe an experiment that you could do to determine whether there are steptomyces colicolor strains producing this drug in the soil samples taken from your backyard.
c) The chip was used to isolate the antibiotic producing the organism. Briefly describe how the ichi works and why it is the device of choice when scientists are trying to isolate antibiotic producing organisms.
d) Briefly describe an experiment that could be done to determine whether the new antibiotic is bacteriostatic or bactericidal.
Answer a:)
To identify the cellular target of the antibody, we must add molecular probe to the antibody. This may be GFP or else that can visible in fluoroscope or in gel electrophoresis.
Answer b:)
To identify the gene encoding the antibiotic in steptomyces colicolor strains collected from the backyard, we must perform hybridization or microarray method.
Answer c:)
The chip must contain such bacteria that are killed by the antibiotic produced by steptomyces colicolor strains. Therefore, after the lethal activity some chemical must change colors of the chip that is visual on its surface. That is how it works.
Answer d:)
The bacteriostatic drug will stop the reproduction of bacteria and hence the solution will still contain live bacteria even after treatment. If the drug is bactericidal, then bacteria will die and release their content. Therefore, in spectrophotometer is we observe relevant OD at 270 nm of a solution, then we will confirm that the drug is bactericidal or otherwise it will be bacteriostatic. This is because DNA absorbs 270 nm wavelength and it will present in the solution when bacteria die.
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You have been hired to study a new antibiotic produced by streptomyces colicolor. The drug has isolated after isolation and identification of streptomyces colicolor using a device known as the chip. y...