Question

shown on Worksheet 27.1), but they will use PCR in- Exercise 2. PCR Can Reveal stead of Southern hybridization analysis. Laboratory workers prepare DNA from samples of Bob's and Mary's blood and then mix a tiny sample Another laboratory wishes to look at differences be of DNA from each person with DNA polymerase en- tween Bob's and Mary's DNAs. They will also look zyme, deoxynucleotides, and the primers shown on at the highly variable region of chromosome 8 Workshect 27.2. The PCR was allowed to proceed Use witb Worksheet 27.2 through 30 replication cycles shown on Worksheet 27.2 (the same region is also Questions Mary's DNA Bob's DNA 1. Write the sequence of the major PCR product from Bob's maternal chromosome. Fragment size, bp Sample wells 2. Write the sequence of the major PCR product from Bob's paternal chromosome. 70 3. Write the sequence of the major PCR products from Mary's maternal and paternal chromosomes Clabel them "maternal" and "paternal"). 60 4. Draw what the products would look like in a 50~ stained gel in the outline provided 40 Because PCR yields so many copies of the major product molecule, it is possible to see the major products by simple staining them, even when there is too little original sample DNA to see in a staine gel. PCR products can also be visualized by hy bridization to a probe, as in Southern analysis. С"

Does anyone know where I can find excercises similar to the one on the photo about molecular biology, or if someone recognize the book of this exercise I will really appreciate it.

Answer 1

These kind of exercises on molecular biology is given in the Book on molecular diagnostics by George Patrinos, wilhelm Ansorge, Philip B.Danielson. 3rd edition .

ebook ISBN- 9780128029886