Question

MATCHING: 4. Molecular biological tools and methodologies are being developed at a blinding pace. Choosing the right tool. therefore. becomes a formidable yet critical task for the successful research seientist. From the following partial list, choose the BEST or MOST APPROPRIATE tool or technique for each of the problems or experiments described Not all |Answers must not be used more than once. answers will be used. 2 points each] A. Autoradiography B. Edman degradation C. FACS (luorescence-activated cell-sorting) D. Hybridoma technology E. G. Mass spectrometry H. Northern hybridization I. Patch clamping J. Polymerase chain reaction K. Pulse-chase technique L. Replica plating M. Sanger dideoxynucleotide DNA sequencing technique N. Scanning electron microscopy O. Shot-gun cloning P. Southern hybridization Q. Sucrose density centrifugation R. Transmission electron microscopy S. X-ray crystallography Purification of mitochondria from a mammalian cell extract Visualization of the three-dimensional surface features of a nuclear membrane Specific identification of actin fibers from among all other eytoskeletal structures in an intact cell In a blood sample, separation of the B-cells from the T-cells and enumeration of each cell type Determine the precise molecular weight of a purified protein Measuring the electrical charge required to open/close a voltage-gated ion channel in a single neuron Determine the amino acid sequence of the first 20 amino acids at the N-teminus of a protein Production of an antibody that will recognize a single molecular structure on a cell surface protein Determination of the three-dimensional structure of a protein Trace the intracellular fate of labeled methionine in a virus-infected cell Creation of a library consisting of a random mixture of 20 kb fragments of the E. coli genome Determination of the guanine and cytosine content of a double-stranded DNA molecule Detection of a gene in a dried blood spot found at the scene of a crime Quantitation of a specific messenger RNA using a labeled DNA probe

Answer 1

Purification of mitochondria from a mammalian cell extract.

In this case, Ion- Exchange chromatography could be applied. This technique is used to separate molecules on the basis of differences in their net surface charge. Molecules on each cell compartment are charged in different ways and this property is used to separate them depending on their overall charge, charge density and surface charge distribution.

Visualization of the three dimensional surface features of a nuclear membrane

Immunofluorescence microscopy is used to visualize different compartments in the cell. In this case is a good tool to visualize nuclear membrane, This will depend on which antibody is used to label the surface and which microscopy is used. It's recommended a confocal microscope.