Question

9. In the context of the technique of gel filtration explain the terms linear range (look it up in the lab manual) and fractionation range. What is the difference in these? 10.One of the assumptions used in the derivation of the Michaelis-Menton equation is that the velocity represents initial velocity. Explain what exactly initial velocity is.

Answer 1

9.In gel filtration, the main aim is to separate the molecules based on the size of the molecules. The beads have a defined pore size range, known as the fractionation range. Molecules and complexes that are too large to enter the pores stay in the mobile phase and move through the column with the flow of the buffer. Linear range is the range upto which a molecule can travel in the bed and it entirely depends on the concentration of the chromatographic column.

10. The assumption is accurate. The initial velocity is the initial rate at which the reaction proceeds. it is assumed to be constant throughout. And that is one of the major drawbacks of the assumption, which is corrected by the Briggs-Haldane assumption.