E. coli strain BL21(DE3) was transformed with the pET30a+ plasmid containing the ilvC gene that encodes for E. coli KARI. The starter culture was incubated overnight in LB broth containing 50 µg/mL kanamycin at 37°C. The sample were already added IPTG and the OD600 reaches the 0.6-0.7 range.
How to improve the purity and yield ?
E. coli strain BL21(DE3) was transformed with the pET30a+ plasmid containing the ilvC gene that encodes for E. coli KARI. The starter culture was incubated overnight in LB broth containing 50 µg/mL ka...