Question

7 6 O No inhibitor Inhibitor A 5 ▲ Inhibitor B 4 3 2 12000 6000 -4000 -2000 2000 4000 8000 10000 1/[S] -1

A biochemist studies the properties of a metabolic enzyme she has just isolated. She obtains kinetic data in the presence and in the absence of two different inhibitors (A and B). The identity of the inhibitors is unknown but we know that one of these is an substrate analog while the other is an alkylating agent.

From the plot above determine:
a) Km and Vmax of the enzyme
b) which inhibitor is the substrate analog? Which is the alkylating agent?
c) Ki for both inhibitors;
d) what would be the Vo for this enzymatic reaction at [S] = 3 x 10-4 M and in the presence of the inhibitor [A] = 2 x 10-5 M?

I have the answer for a) and b) Please show me how to get the answers for c) and d) mathematically not with excel!!

Answer 1

valut ワ し。 ,..] @Mar So max Es k r Puting m Jt caltatim Vm (S)