Describe the structural features and mechanism of assembly of Escherichia coli P (Pap) pili. Use a diagram to illustrate...
Describe the structural features and mechanism of assembly of Escherichia coli P (Pap) pili. Use a diagram to illustrate you answer. When are these pili used during infection, and how is receptor specificity determined in this system? [ESSAY TYPE QUESTION]
Homework Answers
"pap" stands for Pyelonephritis-associated pili. These pilli are encoded by pap genes. They are assembled via conserved chaperone-usher pathway. Pap D is periplasmic immunoglobulin (Ig)–like chaperone and being a chaperone it assists the assembly and delivery of pili subunits to the outer membrane usher PapC. PapA forms helix fiber on the external cell. PapH anchor the pilli to the membrane. Pap E forms tip of the fibrillum and PapG is the adhesin at the end of a tip fibrillum. PapK and PapF are adaptor subunits. Pap A provides a driving force for the translocation of pili subunits across the outer membrane, facilitating outward pilus growth.
It binds to D-galactose-D-galactose residues on red blood cells. and help in moving up the urethra causing urinary tract infections.
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Describe the structural features and mechanism of assembly of Escherichia coli P (Pap) pili. Use a diagram to illustrate...
Topic: Bacteria Chemotaxis (E. coli) 1. You are studying the interaction of CheY and the P1-P2...
Topic: Bacteria Chemotaxis (E. coli)
1. You are studying the interaction of CheY and the P1-P2
domains of CheA. You have used site specific mutagenesis to replace
F214 of CheA with an alanine residue.
A. What genetic experimental assay could you use to determine
the effect of the F214 to A214 substitution on the interaction
between CheA-P1P2 and CheY. Explain how the assay works.
B. What would be the effect of the F214 to A214 substitution on
the interaction between...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
Frederica wants to try using yogurt as the delivery mechanism for her vaccine. She will clone...
Frederica wants to try using yogurt as the delivery mechanism for her vaccine. She will clone a gene from S. pneumoniae and express that gene in Lactococcus lactis, an organisms used to make yogurt. Eating the yogurt would serve as the vaccine delivery mechanism. She even has a name for her new vaccine: SpYogurt! Table 4. Potential vaccine candidates for the prevention of S. pneumoniae infections. gene Protein(s) Strain-to-strain sequence variabilitya Protein location Protein activity ID50 of mutantb plyA Pneumolysin...
please help me. answer only first page. thank you! p. If you accidentally forgot to inoculate...
please help me. answer only first page. thank you!
p. If you accidentally forgot to inoculate your organism in an OF-glucose tube with oil, and only had one yellow “no oil” tube, what type of carbohydrate catabolism would describe that organism (fermenter, oxidative, neither)? Explain your answer. q. What are two other results from the OF-glucose tubes other than their type of carbohydrate catabolism? r. How can organisms that don't use starch grow on a starch agar plate? Carbohydrate Catabolism...
Food Microbiology Purpose To utilize the process of fermentation to make yogurt To describe organisms responsible...
Food Microbiology Purpose To utilize the process of fermentation to make yogurt To describe organisms responsible for food borne illnesses and summarize a recent outbreak Introduction Microorganisms have been used for centuries for food preservation and to improve or change its taste. Evidence exists that yogurt, which is milk fermented by bacteria, has been around for over 4000 years. Today, many of the foods we eat are the result of microorganisms acting on foods for a specific and desired effect....
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate;...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B...
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B by-product of microbial metabolic activities C icorial growth D host-preste relationship E None of the above 2.Microbes muy compete ayust the environment tempera requirements in order to compete for nutrients A True B. False , c.) to the prima A B The pathogenesis of disease can move son of issues by extracellule enzymes or physical blocking of capillaries due to unlimited microbial greneth True...
Need help, numbers 3-6 please show work. and fill out table of chemicals for all chemical...
Need help, numbers 3-6 please show work. and fill out table of
chemicals for all chemical used.
calculate mmol in the table as well show work please
and each of these must be listed in its own row. There are 10 column headings: IUPAC name OR common name, structure, CAS number (Chemical Abstract Service number) molecular mass, melting point (if applicable), boiling point (if applicable), solubility (in water, if applicable), density, amounts to be used in the experiment, and the...
Topic: Bacteria Chemotaxis (E. coli)
1. You are studying the interaction of CheY and the P1-P2
domains of CheA. You have used site specific mutagenesis to replace
F214 of CheA with an alanine residue.
A. What genetic experimental assay could you use to determine
the effect of the F214 to A214 substitution on the interaction
between CheA-P1P2 and CheY. Explain how the assay works.
B. What would be the effect of the F214 to A214 substitution on
the interaction between...
please help me. answer only first page. thank you!
p. If you accidentally forgot to inoculate your organism in an OF-glucose tube with oil, and only had one yellow “no oil” tube, what type of carbohydrate catabolism would describe that organism (fermenter, oxidative, neither)? Explain your answer. q. What are two other results from the OF-glucose tubes other than their type of carbohydrate catabolism? r. How can organisms that don't use starch grow on a starch agar plate? Carbohydrate Catabolism...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B by-product of microbial metabolic activities C icorial growth D host-preste relationship E None of the above 2.Microbes muy compete ayust the environment tempera requirements in order to compete for nutrients A True B. False , c.) to the prima A B The pathogenesis of disease can move son of issues by extracellule enzymes or physical blocking of capillaries due to unlimited microbial greneth True...
Need help, numbers 3-6 please show work. and fill out table of
chemicals for all chemical used.
calculate mmol in the table as well show work please
and each of these must be listed in its own row. There are 10 column headings: IUPAC name OR common name, structure, CAS number (Chemical Abstract Service number) molecular mass, melting point (if applicable), boiling point (if applicable), solubility (in water, if applicable), density, amounts to be used in the experiment, and the...
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