Question

35. Insulin is a polypeptide hormone that contains two short polypeptide chains linked by two interstrand disulfide bonds. In order to sequence the polypeptide chains, the most logical order of events to perform would be: I: Reduce the peptides with mercaptoethanol. II: Sequence the peptides using Edman degradation. III: Separate the peptides by gel filtration. IV: Alkylate the peptides with iodoacetate a) I, IV, III, I 36. Which ONE of the following statements regarding chaperonin is INCORRECT? The majority of E. coli proteins do not interact with chaperonin. a) Unfolded proteins with a molecular weight less than 70 000 Da bind in the central cavity. b) Its core structure has 2 rings and each ring is comprised of 14 different subunits. c) Each subunit binds ATP, which is hydrolyzed during protein release. d) 37. Prolyl hydroxylase requires all of the following EXCEPT: a) ascorbic acid. b) a-ketoglutarate c) molecular oxygen. d) succinate.

Answer 1

35.

I. reduce the peptides with mercaptoethanol

IV. Alkylate the peptides with iodoacetamide

III. Separate the peptides by gel filtration

II. Sequence the peptides using Edman degradation

37.

D. Succinate

36.

B