Homework Answers
concentration of calcium in the sample = 323 ppm
Explanation
concentration of original stock solution = 1000 ppm
concentration of 1:10 diluted stock solution = 100 ppm
| dilution | concentration | absorbance signal |
| 1:20 | 5 ppm | 9.1 |
| 1:10 | 10 ppm | 18.6 |
| 1:5 | 20 ppm | 37.0 |
Equation of best fit line : y = 1.8571x - 0.1
absorbance signal of sample = 23.9 = y
from the equation of best fit line,
x = (y + 0.1) / 1.8571
x = (23.9 + 0.1) / 1.8571
x = 12.9
concentration of diluted original sample = 12.9 ppm
concentration of undiluted original sample = 323 ppm
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