Question

How would a mixture of lysine, cysteine and histidine separate on an anion exchange column, if the procedure included a three stage elution strategy: first step elution with a pH of 9; second step elution with a pH of 7; and a third step elution with a pH of 2. In your answer, include both an explanation (including expected charges/calculations) and a chromatogram (drawing).

Answer 1

Anion exchanse ohmm H E-ve chavse exause NH P1o7 LySme stene pk: 11 tishdnes pki = 1-82 Pk2=g.95 Pki 2.3 NM-どこ Phe=3 33 Pue- 6-04 PAz-917 N =-1 At PH 9 HiS =O L38=キ1 C H3=0 PH=7 tis+ L93ニ+2 Pt= 2 TAAA HES SA7 PH 9 PH-2 PH 7