Weigh out 0.05g of the
starting material to 0.0002g and transfer it to a 500 mL
volumetric flask. Dilute to the mark.
Withdraw 1 mL from the volumetric flask with a volumetric pipet and transfer to a 100mL volumetric flask. Dilute to the mark.
This procedure yields a final concentration of 1.00 ug/mL, as stated above.
Explanation:
As the balance have 0.0002 g sensitivity we can weigh 0.05 g.
If we dissolve 0.05g in 500 mL solvent we will get a concentration of 0.0001 g/mL. Then if we diluted its 1 mL to 100 mL then final concentration will be 0.000001g/mL = 1ug/mL.
analytical balances that are accurate to 0.0002 g; volumetric flasks of 50, 100, 250 and 500 mL; and volumetric pipets...
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