Question

Please help me answer this question for ANALYTICAL CHEMISTRY  subject. PLEASE answer it seriously and completely, or better not answer it if not then, really appreciate it, thank you.

6 For the following samples, suggest the most suitable/appropriate technique that can be used to do the required analysis. The technique can be spectroscopic, chromatographic or a combination of both. Explain why you choose that method/technique (a) Deionised distilled water obtained from an in-house facility has claimed to be "ion- free. A quick analysis is needed to determine up to parts per trillion (ppt) levels the ionic content in the deionised distilled water sample (b) A powdered sample grounded from razor clam shell is suspected to contain many heavy metals originating from its surrounding. The identities and concentration of those heavy metals need to be determined at ppb level (c) Soil sample obtained from a river bed is suspected to be contaminated with various chemicals. The structure and concentration of halogenated compounds in the sample need to be identified and determined at ppb levels tasting property. Globally it is marketed under one of the two isomers of (d) Aspartame is a protein with very sweet trade name Nutrasweet and Equal. However, only aspartame exhibits the sweet tasting characteristics. Synthesis of aspartame resulted in the formation of both isomers. Suggest a technique that can distinguish, identify and quantify the two isomers in a mixture?

Answer 1

6 a) For first process we only need spectroscopic technique to analyze. As there is no other component or it is not a mixture there is no need for chromatography. We just need the spectrophotometer to perform quantitative analysis of ions.

b) In this sample there may be more components so first we have to isolate those then identify for this we have to employ chromatography first to separate those individual heavy metals. Now these heavy metals need to quantify by spectroscopic technique utilizing beer's-lambert's law.

c) In this case also both the chromatographic and spectroscopic technique needs to be applied. First chromatography will separate the contaminated chemicals into individuals and spectroscopy will determine their concentration in ppb range.

d) In this case also both the chromatographic and spectroscopic technique needs to be applied. At first by chromatography stereospecific ressolution required which will employ chiral stationary phase. But for particular quantification we need spectroscopy.