Question

Most Clinical Pathology Labs in the US use Nucleic acid-based pathogen detection methods whenever possible. One such method is Multiplex Polymerase Chain Reaction (PCR). You suspect your patient may have both a bacterial and a viral infection.

(a) What must be kept in mind when carrying out multiplex PCR to distinguish the different bacterial and viral genes aside from designing primers? Hint: fragment.

Answer 1

The following things must be kept in mind when carrying out multiplex PCR to distinguish the bacteria and virus are

• Always each bacteria as a unique DNA which vary from one another
• The virus usually as RNA sequence mostly
• The reverse transcriptase are less frequently used.
• More than one pair of primers can be used in the reaction
• It is used in gene deletion analysis, mutation,FNA detection, quantitative analysis
• The sample of CSF is used in multiplex PCR to rule out most viral infections
• Nested multiplex PCR in the detection and duddifferentiation if viruses
• Spn9802 fragment can be used in differentiating