Please find principle and calculation related to ion exchange chromatography
please explain thoroughly and show all work If you applied an amino acid mixture containing Histidine...
15. Which is used to reduce the disulfide bonds in proteins? D) iodoacetate B) performic acid C) DTT A) PITC 16. What is the net charge on the tripeptide Gly-Asp-Lys at pH 1.5? The table below gives the pKa's of the ionizable groups on the free amino acids. Free amino acid pK, of a-Carboxyl pKs of side chain pKa of a-amino group 2.4 group 9.8 Glycine Aspartate Lysine 2.0 3.9 9.9 2.2 9.1 10.5 A) -2 B) C) D) +2...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala plus lys. anion exchange resin was used Tubes 2,3, 6 and 7 had a spot appear that was light pink We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
Answer the questions using the data and graph. please show all work. Thank you Titration of Hydrochloric Acid: 1) Use the buret that is located near the hydrochloric acid container to dispense 25.00 mL of hydrochloric acid solution into a clean dry 100 mL beaker. Record the molarity of this solution on your data sheet. 2) Check to make sure that the volume on your sodium hydroxide buret reads 0.00 mL. If it does not read 0.00 mL, adjust it...
Please summarise the following content:(no word limit) EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...