Question 4 Part B [20 marks] deBruijn graph (7-mer) reads have been derived: You will use...
Genetics Question 4 Part B [20 marks] You will use a deBruijn graph to assemble the 10bp circular genome from which these short (7-mer) reads have been derived: Reads: GCAGGTA TAACCGC GTAACCG CCGCAGG AGGTAAC A) Break the reads into the 10 k-mers for k 3 that you can obtain from these reads and write them out next to the reads in your book. [5 marks B) Draw a deBruijn graph using the k-mers as the edges to connect k -...
Question 4 Part A [10 marks] A human genome has been re-sequenced using a sequencing technology that produces 100 bp reads of high quality data. The DNA was sheared to make a paired-end library with a size range of 1500 bp +- 500 bp and sequenced to generate paired-end reads. The analysis pipeline takes short-read sequence data and aligns it to a repeat-masked version of the reference human genome sequence assembly using BLASTN. This table summarizes the results from three...