Question

please answer ALL questions completely showing ALL work.

micro liter as if Dee Enay pipetted sub of a 65 mg/ml Solution, how many_mg did She add to her reaction? Q2: How many grams of Ala (S04)18 Hao CMW clé 2 g/mol) would you ack to 200ml water to make a 20mm Stock Solution? 3 ! calculate the concentration of the unknown protein, and explain your work. Include what the dilution factor is ! 0.239 absorbance for 1:100 dilution Q4: You have 5mL of a 3ng/mL protein solution. (This solution is valuable so you don't want to waste it ! How would you makeup 100ML) of a o.1 mg/mL using a convenient dilution micro L

Answer 1

65 mg/mL solution means that it contains 65 mg in 1 mL. The 1L is equal to $10^{6}$ microliter. In other words, 65 mg is present in $10^{6}$ microlite.

The mg contained by 15 microliter is calculated as follows:

10microliter = 65mg

65 1microliter = 106mg

65 15microliter = x 15mg = 9.8 x 10-4mg