Calcium ions bind to the SERCA Ca2+-ATPase, which has two identical calcium sites, in two stages with apparent equilibrium constants K1 = 7 X 105 M-1 and K2 = 2 X 106 M-1. Calculate K and τ, and plot the fraction of sites occupied over the range of calcium concentrations 10-5 M > [Ca2+] > 10-7 M-1. Is the binding positively or negatively cooperative?
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