You are hired to design the front end of an imaging system for studying the boundary shapes of cells, bacteria, viruses, and protein. The front end consists, in this case, of the illumination source(s) and corresponding imaging camera(s). The diameters of circles required to enclose individual specimens in each of these categories are 50,1,0.1, and 0.01 µm, respectively.
(a) Can you solve the imaging aspects of this problem with a single sensor and camera? If your answer is yes, specify the illumination wavelength band and the type of camera needed. By “type,” we mean the band of the electromagnetic spectrum to which the camera is most sensitive (e.g., infrared).
(b) If your answer in (a) is no, what type of illumination sources and corresponding imaging sensors would you recommend? Specify the light sources and cameras as requested in part (a). Use the minimum number of illumination sources and cameras needed to solve the problem.
By “solving the problem,” we mean being able to detect circular details of diameter 50,1,0.1, and 0.01 µm, respectively.
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