In studies of recombination between mutants 1 and 2 from the previous problem, the results shown in the following table were obtained.
Strain | Dilution | Plaques | Phenotypes |
E. coli B | 10–7 | 4 | r |
E. coli K12 | 10–2 | 8 | 1 |
(a) Calculate the recombination frequency.
(b) When mutant 6 was tested for recombination with mutant 1, the data were the same as those shown above for strain B, but not for K12. The researcher lost the K12 data, but remembered that recombination was ten times more frequent than when mutants 1 and 2 were tested. What were the lost values (dilution and colony numbers)?
(c) Mutant 7 (Problem 22) failed to complement any of the other mutants (1-6). Define the nature of mutant 7.
During the analysis of seven rII mutations in phage T4, mutants 1, 2, and 6 were in cistron A, while mutants 3, 4, and 5 were in cistron B. Of these, mutant 4 was a deletion overlapping mutant 5. The remainder were point mutations. Nothing was known about mutant 7. Predict the results of complementation (+ or -) between 1 and 2; 1 and 3; 2 and 4; and 4 and 5.
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