Read Tools of Biochemistry 12A before attempting to work Problems.
Mammalian cells growing in culture were labeled for a long time in [3H] thymidine to estimate the rate of DNA synthesis. The thymidine administered had a specific activity of 3000 cpm/pmol. At intervals, samples of culture were taken and acidified to precipitate nucleic acids. The rate of incorporation of isotope into DNA was 1500 cpm/106 cells/min. A portion of culture was taken for determination of specific activity of the intracellular dTTP pool, which was found to be 600 cpm/pmol.
(a) What fraction of the intracellular dTTP is synthesized from the exogenous precursor?
(b) What is the rate of DNA synthesis, in molecules per minute per cell of thymine nucleotides incorporated into DNA?
(c) How might you determine the specific activity of the dTTP pool?
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