The enzyme alcohol dehydrogenase catalyzes the oxidation of ethyl alcohol by NAD+ to give acetaldehyde plus NADH and a proton:
CH3CH2OH + NAD+ → CH3CHO + NADH + H+
The rate of this reaction can be measured by following the change in pH. The reaction is run in 1 ml 10 mM TRIS buffer at pH 8.6. If the pH of the reaction solution falls to 8.4 after ten minutes, what is the rate of alcohol oxidation, expressed as nanomoles of ethanol oxidized per sec per ml of reaction mixture?
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