Problem

Understanding the energetics of the lactate dehydrogenase reactionThe standard reduction p...

Understanding the energetics of the lactate dehydrogenase reaction

The standard reduction potential for (NAD+/NADH+H+) is -0.320 V, and the standard reduction potential for (pyruvate/lactate) is -0.185 V.

a. What is the standard free energy change, ΔGº’, for the lactate dehydrogenase reaction:

NADH + H+ + pyruvatelactate + NAD+


b. What is the equilibrium constant (Keq) for this reaction?


c. If [pyruvate] = 0.05 mM and [lactate] = 2.9 mM and ΔG for the lactate dehydrogenase reaction = -15 kJ/mol in erythrocytes, what is the (NAD+/NADH) ratio under these conditions?

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Solutions For Problems in Chapter 20