Problem

Biochemistry. Table 1* lists data for the substrate sucrose treated with the enzyme invert...

Biochemistry. Table 1* lists data for the substrate sucrose treated with the enzyme invertase. We want to model these data with a Michaelis–Menten function.

Table 1

s	v
2.92	18.2
5.84	26.5
8.76	31.1
11.7	33
14.6	34.9
17.5	37.2
23.4	37.1

*Institute of Chemistry, Macedonia.

(A) Plot the points in Table 1 on graph paper and estimate Vmax to the nearest integer. To estimate KM, add the horizontal line to your graph, connect successive points on the graph with straight-line segments, and estimate the value of s (to the nearest integer) that satisfies .
(B) Use the constants Vmax and KM from part (A) to form a Michaelis–Menten function for the data in Table 1.
(C) Use the function from part (B) to estimate the velocity of the enzyme reaction when the sucrose is 9 and to estimate the sucrose when the velocity is 32.