Buffers
MATHEMATICAL A catalog in the lab has a recipe for preparing 1 L of a TRIS buffer at 0.0500 M and with pH 8.0: dissolve 2.02 g of TRIS (free base, MW = 121.1 g/mol) and 5.25 g of TRIS hydrochloride (the acidic form, MW = 157.6 g/mol) in a total volume of 1 L. Verify that this recipe is correct.
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