Histologist Stains Tissue with Dye When Lisa received the sample of tissue from s...

Histologist Stains Tissue with Dye

When Lisa received the sample of tissue from surgery, she places it in liquid nitrogen, which has a heat of vaporization of 198 J/g at the boiling point of nitrogen, which is -196 °C. When the tissue is frozen, Lisa places the tissue on a microtome, cuts thin slices, and mounts each on a glass slide. One of the stains that Lisa uses to allow the pathologist to see any abnormalities is Eosin, which has a formula,

In preparing to stain the tissues, Lisa uses solutions of lithium carbonate and sodium bicarbonate. The pathologist examines the tissue sample and reports back to the surgeon that no abnormal cells are present in the margin tissue. No further tissue removal is necessary.