Problem

The emergence of antibiotic-resistant strains of Enterococci and transfer of resistant gen...

The emergence of antibiotic-resistant strains of Enterococci and transfer of resistant genes to other bacterial pathogens have highlighted the need for new generations of antibiotics to combat serious infections. To grasp the range of potential sites for the action of existing antibiotics, sketch the components of the translation machinery (e.g., see Step 3 of Figure), and using a series of numbered pointers, indicate the specific location for the action of the antibiotics shown in the following table.

Antibiotic	Action
1. Streptomycin	Binds to 30S ribosomal subunit
2. Chloramphenicol	Inhibits peptidyl transferase of 70S ribosome
3. Tetracycline	Inhibits binding of charged tRNA to ribosome
4. Erythromycin	Binds to free 50S particle and prevents formation of 70S ribosome
5. Kasugamycin	Inhibits binding of tRNAfmet
6. Thiostrepton	Prevents translocation by inhibiting EF-G