A series of fermentation tubes containing varying concentrations of glucose in a nutrient broth were inoculated with a pure bacterial culture. The concentrations of cells in the broth media were determined after 16 hr of incubation at 37°C. Rates of growth and initial glucose concentrations follow. Plot the growth rate expressed as cell divisions per hour versus initial glucose concentration. Estimate the maximum growth rate and the saturation constant (glucose concentration at one-half the maximum growth rate). Write an equation in the form of Eq. 3-19, and draw the curve for this equation on the graph with the plotted data. Does the growth of this pure culture appear to be a hyperbolic function as defined by the Monod relationship?
Glucose ( moles x 10-4) | Cell Divisions (per hr) | Glucose ( moles x 10-4) | Cell Divisions (per hr) |
0.1 | 0.23 | 0.8 | 0.94 |
0.1 | 0.28 | 1.6 | 1.06 |
0.2 | 0.32 | 3.2 | 1.15 |
0.4 | 0.71 |
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