Determine the amino acid sequence of a polypeptide from the following data:
Acid hydrolysis gives Ala, Arg, His, 2 Lys, Leu, 2 Met, Ser, Thr, Val.
Carboxypeptidase A releases Val.
Edman’s reagent releases PTH-Leu.
Cleavage with cyanogen bromide gives three peptides with the following amino acid compositions:
1. His, Lys, Met
2. Thr, Val
3. Ala, Arg, Leu, Lys, Met, Ser
Trypsin-catalyzed hydrolysis gives three peptides and a single amino acid:
4. Arg, Leu, Ser
5. Met, Thr, Val
6. Lys
7. Ala, His, Lys, Met
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