Proteins with long peptides can be cleaved into smaller fragments by using certain chemicals that cleave at specific sites on the amino acid residues. Some of these chemicals include Cyanogen Bromide that cleaves proteins after methionine side chains.
In chemical fragmentation, certain chemicals cause proteolysis Cyanogen bromide is used to specifically cleave the methionine residues at the C-terminal.
(a)
Cyanogen Bromide will cleave the carboxyl terminal of methionine in the peptide. The given protein has amino acid sequence DSRLSKTMYSIEAPAKLDWEQNMAL.
The peptide when treated with Cyanogen Bromide, it would make cuts at the carboxyl group of methionine forming a homoserine lactose.
Chemical fragmentation with Cyanogen bromide would give the following fragments:
Thus, number of peptide fragments formed after cleaving with cyanogen bromide is .
The proteolysis with Cyanogen bromide would make two cuts at the C-terminal of Methionine in the peptide and gives 3 fragments DSRLSKTM, YSIEAPAKLDWEQNM, and AL.
(b)
Trypsin will hydrolyze the peptide bonds after lysine and arginine. The given protein has amino acid sequence DSRLSKTMYSIEAPAKLDWEQNMAL.
The peptide when treated with Trypsin, it would make cuts at the carboxyl group of arginine and lysine unless followed by proline.
Enzymatic proteolysis with Trypsin would give the following fragments:
Thus, the number of peptide fragments formed after cleaving with Trypsin is .
The fragmentation with Trypsin would make three cuts at the C-terminal of Arginine and Lysine in the peptide and gives 4 fragments DSR, LSK, TMYSIEAPAK, and LDWEQNMAL.
(c)
Fragmentation with Cyanogen Bromide would yield peptide with 3 fragments. The protein DSRLSKTMYSIEAPAKLDWEQNMAL when treated with Cyanogen Bromide it makes a cut after the methionine group, forming the smallest fragment with only two amino acids.
When Cyanogen Bromide cuts the protein after methionine, then a small fragment is formed having amino acids A and L, which is as following:
DSRLSKTM-YSIEAPAKLDWEQNM-
When the peptide is treated with Trypsin, it would make cuts after amino acids lysine and arginine giving 4 fragments.
Thus, the smallest single fragment with only 2 amino acid residues is formed by Cyanogen Bromide.
The protein when treated with Trypsin, it would give the following fragments:
DSR-LSK-TMYSIEAPAK-LDWEQNMAL
Here four fragments are formed and the small fragment has three amino acids. When compared to the peptide cleaved with Trypsin, the peptide hydrolyzed by Cyanogen Bromide, has smallest fragment with only two amino acids.
Thus, number of peptide fragments formed after cleaving with cyanogen bromide is .
Thus, the number of peptide fragments formed after cleaving with Trypsin is .
Thus, the smallest single fragment with only 2 amino acid residues is formed by Cyanogen Bromide.
Thus, number of peptide fragments formed after cleaving with cyanogen bromide is .
Thus, the number of peptide fragments formed after cleaving with Trypsin is .
Thus, the smallest single fragment with only 2 amino acid residues is formed by Cyanogen Bromide.
How many peptide fragments would result from cleaving the sequence with...
anyone can help me with it? A protein biochemist attempted to determine the amino acid sequence of a decapeptide. Use the results from the trypsin, chymotrypsin, and cyanogen bromide treatments to suggest the amino acid sequence of this decapeptide. Trypsin digestion gave two fragments with multiple residues (not in order): • T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr • T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CT1: Ala, Phe •...
You have a peptide whose sequence is secretly known, that you want to analyze. The peptide sequence is D-M-K-T-L-A-R-S-M-E-I-D-Q You have three reagents that cleave polypeptides, CNBr, chymotrypsin, and trypsin into smaller peptides. If you could purify these peptides and sequence them by Edman, but only get four amino acids. 1) what end of the protein does the Edman reaction cleave residues from? n-terminus or c-terminus 2) what are all the Edman sequences (up to 4 amino acids) of the...
please help!!! 19. A peptide is composed of the following amino acids: Ala, Arg. Glu, Gly, Lys, Met, Phe, Ser, Tyn Given the following data deduce the sequence of the peptide. Trypsin digestion yielded two fragments with the following composition: Fragment 1: Ala, Arg, Glu, Met, Phe, Ser Fragment 2: Gly, Lys, Tyr Chymotrypsin digestion yielded three fragments with the listed compositions: Fragment 1: Ala, Gly, Lys, Phe Fragment 3: Arg, Glu, Met, Ser Cyanogen Bromide cleavage yielded two fragments...
Please be clear with how you determined the code sequence and isoelectric point 7. A single 14 amino acid peptide chain is to be assigned from the following experimental information below, your job is to provide the full triple letter code sequence (if possible), calculate its isoelectrie point and show your logie behind your answer. (12 marks) Experimental information: (i) Amino acid analysis: Cx2, Exl, Fxl, Wx1, Gx1, Hx1, Axl, Lx1, Dx2, Rx1, Qxl, and Nxl. (ii) Chymotrypsin Digestion of...
Need help with #2 Clearly provide the 3-letter symbol for the correct sequence of cach amino acid in each of listings of the amino acids in the peptide or in any fragments are simply listed in alphabetical order. A subscriptive the number of that amino acid. Use parentheses to incorporate the unknown amino acids after each treatment 1. A five amino acid peptide contains: Arg. Glu, His, Phe, and Ser This peptide yields the following results when broken down into...
How to do this problem? 1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys, Phez, Serz, Trp, Tyr, Val This peptide yields the following results when broken down into smaller fragments Please note the AA are given in alphabetical order). Show your work, explain your reasoning, give the answer in three letter abbreviation and one letter abbreviation Fragment Content His Treatment Edman Chymotrypsin Gly. His, Phe Ser, Tyr Arg2, Leu, Lys, Ser.Ala, Phe Trp, Val Gly, His, Lys,...
How to do this problem? 1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys, Phez, Serz, Trp, Tyr, Val This peptide yields the following results when broken down into smaller fragments Please note the AA are given in alphabetical order). Show your work, explain your reasoning, give the answer in three letter abbreviation and one letter abbreviation Fragment Content His Treatment Edman Chymotrypsin Gly. His, Phe Ser, Tyr Arg2, Leu, Lys, Ser.Ala, Phe Trp, Val Gly, His, Lys,...
6. The sequence of kassinin, a tachykinin dodecapeptide from the African frog Kassina senegalensis, was determined. A single round of Edman degradation identifies Asp as the N-terminus. A second sample of the peptide is treated with chymotrypsin. Two fragments are released with the following amino acid compositions: fragment I (Gly, Leu, Met, Val) and fragment II (Asp2, Gln, Lys, Phe, Pro, Ser, Val). Next, a third sample of peptide is treated with trypsin, which results in two fragments with the...
7. A peptide was isolated from sheep hypothalamus and subjected to sequence analysis. The experimental data are as follows. Deduce the amino acid sequence from this data. a. Composition (2Ala, 2Gly, Thr, Phe, Lys, His, Ser, Trp, Arg, Val) b. Reaction of dinitrofluorobenzene with the intact peptide yielded DNP-Thr. A brief treatment with carboxypeptidase a yielded glycine. c. Trypsin digestion yielded three fragments: i. Phe, Thr, Ala, Lys ii. Gly, Val iii. Trp, His, Ser, Gly, Arg, Ala Treatment of...
In part A, yes you should reconstitute the full-length protein sequence from the fragments. However, you do not need to write out the amino acid sequence - you can just provide the order of the fragment numbers (from either set of fragments). For example (and this is not the answer), you could say: "Using the Pro-IAPP fragment set, starting from the N-terminus, the fragment order is '5-4-6-3-2-1' " and that would fully address the question. In part B, the hint...