a solution X of concentration 0.010mol dm gives an absorbance of 0.5. What concentration is a solution of x which gives...
a solution X of concentration 0.010mol dm gives an absorbance of 0.5. What concentration is a solution of x which gives an absorbance reading of 0.25? Assume that the same optical cell is used for both readings
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a solution X of concentration 0.010mol dm gives an absorbance of 0.5. What concentration is a solution of x which gives...
A 1.53M solution of "A" has an absorbance of 0.45. What is the absorbance of solution "B" which h...
A 1.53M solution of "A" has an absorbance of 0.45. What is the absorbance of solution "B" which has a concentration of 0.88M? The path length of the cell is 3.0cm. ( Write answer to three decimal places, if answer is 1.7654 then answer should be entered as 1.765)
The Absorbance for the highest concentration of starch is lower than you might have expected. What...
The Absorbance for the highest concentration of starch is lower than you might have expected. What is the most likely reason for this? The sample was prepared incorrectly and actually has a lower starch concentration than indicated. Not enough Lugols was added, and not all of the starch is being detected. The measurement was made inaccurately, and the Absorbance is probably higher than indicated. Spectrophotometer readings are inaccurate near their maximum value of 2. You have series of starch solutions...
The above graph gives the absorbance of vitamin B, solution in water, in a 1.00 cm...
The above graph gives the absorbance of vitamin B, solution in water, in a 1.00 cm cell, at 362 nm. Given the information on this graph, answer the following question. What is the molar extinction coefficient of B,, (aq) at 362 nm? 0.180 0.160 y = 0.0217x R2 = 0.8092 Ø 0.140 $0.120 Absorbance (no units) 0.060 0.040 0.020 0.000 0.000 8.000 2.000 4.000 6.000 Concentration (um)
What is the range of optical density that can be used to reliably convert absorbance to...
What is the range of optical density that can be used to reliably convert absorbance to cell concentration
4, If a 0.1mM solution of a protein gave an absorbance of 1 absorbance unit. What...
4, If a 0.1mM solution of a protein gave an absorbance of 1 absorbance unit. What is the concentration of a solution of this protein that after being diluted 1000 times gave an 280nm of 0.5 Absorbance units. absorbance at + ο.0001 OmM>A
Solution 1 with a concentration of 0.31M had an absorbance of 1.42. Solution 2 had an absorbance of 0.62, what is the co...
Solution 1 with a concentration of 0.31M had an absorbance of 1.42. Solution 2 had an absorbance of 0.62, what is the concentration of solution 2? The path length is 2.00cm.
4. At 25°C, the resistance of a conductivity cell with KCI solution (0.01 mol dm) is...
4. At 25°C, the resistance of a conductivity cell with KCI solution (0.01 mol dm) is 112.10. After filling another solution X with the same concentration, its resistance changes to 2184 (5 marks) (1) Calculate the cell constant Ke (2) Calculate the conductivity of X solution, (3) Calculate the molar conductivity of X solution. (Ignore the water conductivity, At 25°C, the conductivity of KCI solution (0.01 mol dm) is 0.14114 Sm')
Page 9 Questions 1. A substance exhibits an absorbance of 0.47 in a 1.00 cm cell...
Page 9 Questions 1. A substance exhibits an absorbance of 0.47 in a 1.00 cm cell at 525 nm. (a) What will be its absorbance in a 2.7 cm cell? (b) If the substance concentration is 2.1 x 10-5 mol dm, compute its molar absorptivity. (c) What absorbance will it have in a 5.0 cm cell at 2. = 405 nm (think about it!)? 2. You are setting up an absorption spectrometer to determine the concentration of a gas which...
6) According to the Beer-Lambert Law, what would happen to the absorbance of a solution if...
6) According to the Beer-Lambert Law, what would happen to the absorbance of a solution if the concentration of analyte (substance being studied) is tripled? 7) At a wavelength of 447 nm, the absorbance of a solution that is 5X10M in the FeSCN complex is found to be 0.981. Assuming this is accurate, calculate the molar absorption coefficient (absorptivity) for the complex if the optical path length is 1.0 cm.
Name: Determination of Solution Concentration by Spectrophotometry PRELAB QUESTIONS 1. A student measures the absorbance of...
Name: Determination of Solution Concentration by Spectrophotometry PRELAB QUESTIONS 1. A student measures the absorbance of an unknown after placing a sample in a cuvette which contained some water. How will this affect the concentration he reports? 2. Explain why it is all right to leave water in the volumetric flask before you put in the 10.00 mL of your unknown. 3. A 1.3 x 10-M solution of an unknown substance has an absorbance of 0.345 at its max. a)...
The Absorbance for the highest concentration of starch is lower than you might have expected. What is the most likely reason for this? The sample was prepared incorrectly and actually has a lower starch concentration than indicated. Not enough Lugols was added, and not all of the starch is being detected. The measurement was made inaccurately, and the Absorbance is probably higher than indicated. Spectrophotometer readings are inaccurate near their maximum value of 2. You have series of starch solutions...
The above graph gives the absorbance of vitamin B, solution in water, in a 1.00 cm cell, at 362 nm. Given the information on this graph, answer the following question. What is the molar extinction coefficient of B,, (aq) at 362 nm? 0.180 0.160 y = 0.0217x R2 = 0.8092 Ø 0.140 $0.120 Absorbance (no units) 0.060 0.040 0.020 0.000 0.000 8.000 2.000 4.000 6.000 Concentration (um)
4, If a 0.1mM solution of a protein gave an absorbance of 1 absorbance unit. What is the concentration of a solution of this protein that after being diluted 1000 times gave an 280nm of 0.5 Absorbance units. absorbance at + ο.0001 OmM>A
4. At 25°C, the resistance of a conductivity cell with KCI solution (0.01 mol dm) is 112.10. After filling another solution X with the same concentration, its resistance changes to 2184 (5 marks) (1) Calculate the cell constant Ke (2) Calculate the conductivity of X solution, (3) Calculate the molar conductivity of X solution. (Ignore the water conductivity, At 25°C, the conductivity of KCI solution (0.01 mol dm) is 0.14114 Sm')
Page 9 Questions 1. A substance exhibits an absorbance of 0.47 in a 1.00 cm cell at 525 nm. (a) What will be its absorbance in a 2.7 cm cell? (b) If the substance concentration is 2.1 x 10-5 mol dm, compute its molar absorptivity. (c) What absorbance will it have in a 5.0 cm cell at 2. = 405 nm (think about it!)? 2. You are setting up an absorption spectrometer to determine the concentration of a gas which...
6) According to the Beer-Lambert Law, what would happen to the absorbance of a solution if the concentration of analyte (substance being studied) is tripled? 7) At a wavelength of 447 nm, the absorbance of a solution that is 5X10M in the FeSCN complex is found to be 0.981. Assuming this is accurate, calculate the molar absorption coefficient (absorptivity) for the complex if the optical path length is 1.0 cm.
Name: Determination of Solution Concentration by Spectrophotometry PRELAB QUESTIONS 1. A student measures the absorbance of an unknown after placing a sample in a cuvette which contained some water. How will this affect the concentration he reports? 2. Explain why it is all right to leave water in the volumetric flask before you put in the 10.00 mL of your unknown. 3. A 1.3 x 10-M solution of an unknown substance has an absorbance of 0.345 at its max. a)...
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