Question

Enzyme Specificity Are Sucrase and Lactase specific to Sucrose and Lactose? I: Experimental Procedure 1. Add 0.3 g of sucrose to three different test tubes. Make sure to remember to label them 1 to 3. 2. Add 5 mL of Dl water to test tube number 1. Add 5 mL of sucrase to test tube 2. Add 5 ml of lactase to test tube 3. 3. Add 0.3 g of lactose to three different test tubes. Make sure to remember to label them 4 to 6. 4. Add 5 mL of DI water to test tube number 4. Add 5 mL of sucrase to test tube 5. Add 5 mL of lactase to test tube 6. 5. Place all six test tubes in a hot water bath at 35-40 °C for 30 minutes, approximately mimicking the temperature of your body. You can make a water bath by taking a large 400-600 ml beaker and fill it up to about % full with tap water. Then place the beaker on a hot plate and begin heating 6. While your test tube samples are incubating in the hot water bath, take "pea size" sample of glucose and mix it with 1 mL of water in an additional test tube. This step is going to allow you to witness a positive test for glucose. After mixing the glucose and water, take a glucose test strip and dip it in the glucose solution and wait 1 minute. Take the glucose test strip out and wait a couple of minutes for the test result to develop. Once you witness the color change of the test strip, note the color and concentration and record the results in part II

please answer questions 1 through 6 with the information provided.

Answer 1

Question 1.

When sucrose is hydrolyzed, the products are glucose and fructose.

When lactose is hydrolyzed, the products are glucose and galactose.

Question 2.

Disaccharides are converted into monosaccharides, which indicates that sucrose and lactose have gone through hydrolysis.

Question 3.

Hydrolysis of sucrose is seen in the presence of an enzyme called invertase.

Question 4.

Hydrolysis of lactose is seen in the presence of an enzyme called lactase.