Question

carbohydrates and enzymes post lab questions

Paragraph Styles Part A observations (salution appearance, test strip colors, etc.) Test tube Glucose levels Initial: 1700 mg/dLInitial: Dark brown Final:1700 mg/dL Final: Dark brown (glucose) Initial:80 mg/dL Initial: light blue with a very slight light green tint M Final: 250 mg/dL Final: Light green (maltose) Initial: 0 mg/dL Initial: light blue Final:250 mg/dL Final: light green (lactose) Initial:0 mg/dL Initial: light blue Final:260 mg/d Final: light green (lactose, acidic) Initial:0 mg/dL Initial: light blue Final: light green with slight brown color Lh (lactose, basic) Final:350 mg/dL Part B Barfoed's test observations (presinitate, color, amount, consistency, etc.) Test tube Initial: light blue clear liquid Final: foggy light blue, resembles melted blue slushy (glucose) Initial: light blue clear liquid MFinal: blue liquid, looks similar to blue bubble blowing lhquid (maltose) Initial: Light blue clear liquid Final: Dark blue, kinda foggy (lactose) Initial.light blue clear liquid L-A (lactose, acidic) Final: dark blue, clear liquid, thin consistency Initial: light blue clear liquid Final: blue, slightly foggy, looked very similar to M (lactose, basic) Postlaboratory Questions The following all answers to the correct number of significant figures. questions must be answered in complete sentences in order to receive full credit Record each answer in the space provided. Show all work for ful credit, include units, and report 1. Identify the solutions that had their glucose levels change after the addition of the lactase enzyme. Do these results make sense? Are there any deviations from what you expected? Explain. 2. Identify which solutions yielded a positive result for Barfoed's test (brick-red precipitate) Indicate what the positive result means and whether the obtained results were expected 3. If you were given three unlabeled samples of glucose, maltose, and lactose, how could you use the results from this lab to identify each unknown? Support your answer with observations from both tests- glucose strips and Barfoed's test 4. Maltase is another enzyme that functions with similar specificity to maltose as lactase does to lactose. Would the results for your five solutions be the same if we used a maltase pill instead of lactase? Would you expect the same levels of glucose after a maltase pill addition? Explain any similarities or differences you anticipate. 5. Starches and fiber are two other sources of carbohydrates. How do they differ from sugars? 5. Starches and fiber are two other sources of carbohydrates. How do they differ from sugars? 6. Which pH condition (acidic, basic, or neutral) resulted in the most successfiul conversion of lactose to glucose? Does the enzyme activity cease entirely, slow down, or remain the same when the pH changes? Use your results to explain Copyright 0 2019 by the Department of Chemistry & Bliochemistry at The Ohio State University 4

Answer 1

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