Question

In comparing fluorescence spectroscopy to UV‑Vis absorption spectroscopy, which of the statements is true? Fluorescence measurements do not suffer deviations from linearity like absorbance measurements do. Fluorescence measurements are more precise and less noisy because only the intensity of the emitted light is measured. Fluorescence instruments are less expensive than absorption measurements. Fluorescence measurements have better detection limits because at low concentrations a low intensity emission signal is being measured against a low intensity background signal.

Answer 1

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