Question

Sea elegans, Three classes of scd-3 alleles available. While recessive sdc-3(null) alleles affect both sex determination and dosage compensation, rare recessive mutations (the sdc-3(Tra) alleles) cause sex determination but not dosage compensation defects. Conversely, rare recessive sdc-3(Dpy) alleles cause dosage compensation defects but not sex determination defects. sdc-3(Tra) and sdc-3(Dpy) complement one another and are very tightly linked to each other. 7a) (2 points) Using only the three alleles described above, how could you demonstrate that Tra and Dpy classes of mutations are actually alleles of the same gene even though they complement? Just show the genotype you would want to look at – don’t worry about how you would construct them. 7b) (1 point) What do you think the Tra and Dpy alleles tell you about the molecular structure of the sdc-3 product?

Answer 1

7b,multiple mutants alleles carried in one strain by chromosomes.. it's a complicated genetic background between dosage compensation and sex determination process to the sdc-3 null background..sdc -3 gene have connection between dosage compensation and sex determination..in sea- elegant sexual fate influenced by a distruption in dosage compensation..downstream genes like tra or dosage compensation dpy gene promotes negative regulating the sdc genes..sdc 1and 2 activity promote hermaphrodite development..it provides negative male development and it's achieving the hermaphrodite specific dpy genes that implement dosage compensation..sdc 1and 2 genes shows single mutation..

Sdc -3 alleles disrupt sex determination that disrupt dosage compensation cause XX specific lethality..it shows no signs of sexual transformation..