35. Plot Lineweaver-Burk Plots (1/V vs. 1/S1) for the equations shown below and show how it...

Question

Question

35. Plot Lineweaver-Burk Plots (1/V vs. 1/S1) for the equations shown below and show how it changes with increasing inhibitor

science chemistry

Add a comment Improve this question Transcribed image text

Answer 1

Answer #1

35. Line we polt for enzyane kinetics with out he weaver-Burk innitition is given by t = kws. t * Vimax (4) Vyax VS now the g

Add a comment

Answer 2

Similar Homework Help Questions

35. Plot Lineweaver-Burk Plots (1/V vs. 1/S1) for the equations shown below and show how it...

35. Plot Lineweaver-Burk Plots (1/V vs. 1/S1) for the equations shown below and show how it changes with increasing inhibitor concentration [1]. Name the type of inhibition. take an ( 1 + LI1 ) [s+ T max
The Lineweaver-Burk plots shown below are for enzyme catalyzed reactions. The reaction without and inhibitor is...

The Lineweaver-Burk plots shown below are for enzyme catalyzed reactions. The reaction without and inhibitor is shown in blue. The reaction with an inhibitor is shown in red. Identify the type of inhibition in each plot. with I with I 1/vo without I without I 1/[S] 1/[S] with without I without I 1/[S] 1/[S] with I without I 1/[S] Problem 4 For each plot above describe how Km and Vmax are affected by the inhibitor.
Write the equations that describe the Michaelis-Menten and the Lineweaver-Burk double-reciprocal plots. Draw examples of each...

Write the equations that describe the Michaelis-Menten and the Lineweaver-Burk double-reciprocal plots. Draw examples of each plot, demonstrating how Km and Vmax can be determined. On the same graphs, draw another plot where the same enzyme-catalyzed reaction is subjected to inhibition by a competitive inhibitor.

A plot of 1/V versus 1/[S], called a Lineweaver-Burk or double-reciprocal plot, is a useful tool...

A plot of 1/V versus 1/[S], called a Lineweaver-Burk or double-reciprocal plot, is a useful tool for identifying the type of enzyme inhibition. Modify each graph by dragging the endpoints to show the various types of enzyme inhibition. Competitive inhibition What is the inhibition mechanism for the competitive inhibitor? with inhibitor UNI The inhibitor binds to both free enzyme and enzyme-substrate complexes with identical binding constants. The inhibitor binds only to free enzyme. The inhibitor binds to both free enzyme...
12. From the Lineweaver-Burk graph given: a. What are the Km and Vmax for the uninhibited...

12. From the Lineweaver-Burk graph given: a. What are the Km and Vmax for the uninhibited case? b. What type of inhibition is occurring? c. Shown on the page after the Lineweaver-Burk plot are semi-qualitative sketches for three V vs. (S) plots. Which one of these most likely corresponds to this particular experiment (A,B or C)? Scani explain th Cant catimer IIIIIIIII IIIIIIIII - -- | - | IIIII We were unable to transcribe this image
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots....

Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel: On the M-M Plot: estimate Vmax, KM On the L-B Plot: determine Vmax, KM, keat, kcat/Km. The total enzyme concentration is 5 uM. Graphs can be 1/2 page. Must be computer generated with all axes labeled. Substrate (mM) V. (mM/s) | 1/[S] (mM1) 1/V. (s/mM) 10 | 0 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228...

1. Graphic depictions of enzyme kinetic data can aid in evaluation of reversible enzyme inhibition. Please...

1. Graphic depictions of enzyme kinetic data can aid in evaluation of reversible enzyme inhibition. Please use the data below to determine type of inhibition for using two different concentrations (in blue and purple). Be sure to include graphical representation (Michaelis-Menton, Lineweaver-Burk plots), type of inhibition, appropriate kinetic schemes and rate equations. The enzyme concentration at its initial state E, was kept constant. All data points are given in arbitrary units. Completely justify your answer. Use Excel or graphing paper...
show work please! with inhibitor (umoles/min/mg) [S] (mm) v- no inhibitor (umoles/min/mg) V - with inhibitor...

show work please! with inhibitor (umoles/min/mg) [S] (mm) v- no inhibitor (umoles/min/mg) V - with inhibitor (umoles 3.0 2.29 x 103 1.83 x 103 5.0 or ble 3.20 x 103 Boristeder s obre o 2.56 x 103 7.0 3.86 x 103 USD 3.09 x 103 0 9. 4 .36 x 100W 3.49 x 103 0 11. 4 .75 x 108 3.80 x 103 Draw Lineweaver-Burk plots for the enzyme data shown above. When present, I = 0.200 m a) What...
3. Why is an allosteric enzyme more sensitive to substrate concentration around Km values than a...

3. Why is an allosteric enzyme more sensitive to substrate concentration around Km values than a Michaelis-Menten enzyme with the same Vmax? 4. Explain how pH and temperature influence enzyme activity. ( A Lineweaver-Burk (double reciprocal) plot was used to compare the effects of three different reversible inhibitors (A, B and C) on an enzyme. The plot of 1/V vs 1/[S] for the enzyme with no inhibitor is shown in a solid black line. The plot of 1/V vs 1/[S]...

1 Graphic depictions of enzyme kinetic data can and in evaluation of reversible enzyme inhibition. Please...

1 Graphic depictions of enzyme kinetic data can and in evaluation of reversible enzyme inhibition. Please use the data below to determine type of inhibition for using two different concentrations (in blue and purple). Be sure to include graphical representation (Michaelis-Menton. I Lineweaver-Burk plots), type of inhibition, appropriate kinetic schemes and rate equations. The enzyme concentration at its initial state E, was kept constant. All data points are given in arbitrary units. Completely justify your answer. Use Excel or graphing...

35. Plot Lineweaver-Burk Plots (1/V vs. 1/S1) for the equations shown below and show how it...

Homework Answers