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paper chromatography
T FOR EXPERIMENT 37 (continued) NAME QUESTIONS AND PROBLEMS 1. Why did you use...
Why did you use a pencil to mark the paper and not a pen? 1. 2....
Why did you use a pencil to mark the paper and not a pen? 1. 2. Why do you have to use a different capillary tube for each sample? Why is it very important in the amino acid chromatogram not to touch the paper with your fingers or hands? 3. 4. If two samples have identical R values does this mean that they are necessarily identical molecules? Explain What is your unknown? How do you know?
6. Other than a liquid solvent, what could function as the mobile phase in chromatography? 7. Why was it necessary to treat the chromatogram with DMG and ammonia? 8. Why did you use pencil to dra...
6. Other than a liquid solvent, what could function as the mobile phase in chromatography? 7. Why was it necessary to treat the chromatogram with DMG and ammonia? 8. Why did you use pencil to draw the bottom line instead of ink? 9. What caused the streaking of color near the staple in the chromatograms? 10. What would you observe on a chromatogram if two ions in your unknown had very similar Rf values?
6. Other than a liquid solvent,...
paper chromatography 2. In each part, A and B, what was the mobile phase and what...
paper chromatography
2. In each part, A and B, what was the mobile phase and what was the stationary phase? CH.- -06 -COON PROCEDURE Wear protective game NOTE: Aller starting Part Aberin Parts over Dispose of all material in the con 1 the vent front to travel up the the Food Color Separation ontainer provide 1. Cut a piece of Whatman human er paper into a 14 cm draw a thin me across the wheel 2 em from one edge...
Background In this lab, we are going to learn how to use a powerful separation technique...
Background In this lab, we are going to learn how to use a powerful separation technique called chromatography which can separate compounds that are quite similar to one another. Chromatography is a general type of separation in which a mixture of compounds passes through a stationary phase. Different compounds have different relative affinities for the mixture traveling along the stationary phase, or the mobile phase, and the stationary phase, causing these compounds to separate from one another. More specifically, we’re...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
1-4 1. What would happen if you used a water-soluble pen on the chromatography paper to...
1-4
1. What would happen if you used a water-soluble pen on the chromatography paper to mark your origin line? How would this affect the Re values? 2. A student accidently adds 17 ml of NaCl to their beaker rather than the 7 mL of NaCl they were suppose to add to their beaker. How might this mistake affect their chromatogram? Do you think they would be able to obtain any data? Briefly explain your answer. 10 3. Is it...
there's a ruler measure Student Name Partner's Name(s) Date Course/Section Instructor EXPERIMENT #6 PAPER CHROMATOGRAPHY LABORATORY...
there's a ruler measure
Student Name Partner's Name(s) Date Course/Section Instructor EXPERIMENT #6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. This marks the distance the mobile phase traveled during elution Pheophytin Xanthophyll Chlorophyll A Chlorophyll B Lutein Poorly resolved Band(s) This circle marks the position the mixture was deposited before elution Table 1. Retention factors for components of leaf extract. Component IR...
Pre-Laboratory Questions SHOW ALL WORK TO RECEIVE CREDIT. 1. What is the purpose of the mobile...
Pre-Laboratory Questions SHOW ALL WORK TO RECEIVE CREDIT. 1. What is the purpose of the mobile phase in chromatography? 2. What is the Ry value for a component in a sample? Lab 3: Chromatography 3. A student developed chromatograms for pure substances A, B, and C, and an containing some of the substances A, B and C. Calculate the values of A, , component of the unknown mixture. Identify the substances present in the unknown pure substances A, B, and...
Post-Lab Questions 1. Why must you use a pencil, and not ink or felt-tip pen, to...
Post-Lab Questions 1. Why must you use a pencil, and not ink or felt-tip pen, to mark on the paper the origin of the spots of the amino acid preparations? 2. In this experiment, suppose you forgot to mark the position of the solvent front when you tested the hydrolysate of aspartame against the other amino acids. a. Is it still possible to determine how many amino acids were present in the hydrolysate? Explain your answer. b. Could you still...
Why did you use a pencil to mark the paper and not a pen? 1. 2. Why do you have to use a different capillary tube for each sample? Why is it very important in the amino acid chromatogram not to touch the paper with your fingers or hands? 3. 4. If two samples have identical R values does this mean that they are necessarily identical molecules? Explain What is your unknown? How do you know?
6. Other than a liquid solvent, what could function as the mobile phase in chromatography? 7. Why was it necessary to treat the chromatogram with DMG and ammonia? 8. Why did you use pencil to draw the bottom line instead of ink? 9. What caused the streaking of color near the staple in the chromatograms? 10. What would you observe on a chromatogram if two ions in your unknown had very similar Rf values?
6. Other than a liquid solvent,...
paper chromatography
2. In each part, A and B, what was the mobile phase and what was the stationary phase? CH.- -06 -COON PROCEDURE Wear protective game NOTE: Aller starting Part Aberin Parts over Dispose of all material in the con 1 the vent front to travel up the the Food Color Separation ontainer provide 1. Cut a piece of Whatman human er paper into a 14 cm draw a thin me across the wheel 2 em from one edge...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
1-4
1. What would happen if you used a water-soluble pen on the chromatography paper to mark your origin line? How would this affect the Re values? 2. A student accidently adds 17 ml of NaCl to their beaker rather than the 7 mL of NaCl they were suppose to add to their beaker. How might this mistake affect their chromatogram? Do you think they would be able to obtain any data? Briefly explain your answer. 10 3. Is it...
there's a ruler measure
Student Name Partner's Name(s) Date Course/Section Instructor EXPERIMENT #6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. This marks the distance the mobile phase traveled during elution Pheophytin Xanthophyll Chlorophyll A Chlorophyll B Lutein Poorly resolved Band(s) This circle marks the position the mixture was deposited before elution Table 1. Retention factors for components of leaf extract. Component IR...
Pre-Laboratory Questions SHOW ALL WORK TO RECEIVE CREDIT. 1. What is the purpose of the mobile phase in chromatography? 2. What is the Ry value for a component in a sample? Lab 3: Chromatography 3. A student developed chromatograms for pure substances A, B, and C, and an containing some of the substances A, B and C. Calculate the values of A, , component of the unknown mixture. Identify the substances present in the unknown pure substances A, B, and...
Post-Lab Questions 1. Why must you use a pencil, and not ink or felt-tip pen, to mark on the paper the origin of the spots of the amino acid preparations? 2. In this experiment, suppose you forgot to mark the position of the solvent front when you tested the hydrolysate of aspartame against the other amino acids. a. Is it still possible to determine how many amino acids were present in the hydrolysate? Explain your answer. b. Could you still...
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