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paper chromatography T FOR EXPERIMENT 37 (continued) NAME QUESTIONS AND PROBLEMS 1. Why did you use pencil to mark the paper and not a pen? 2. In each part, A and B, what was the mobile phase and what was the stationary phase? 3. If two samples have identical Re values does this mean that they are necessarily identi- cal molecules? Explain.
paper chromatography 4. For the green food color, how would it be possible to show that the component spots you separated do produce a green food color? 5. Why did you chromatograph a mixture containing all four of the amino acids? 6. Why is it important in the amino acid chromatogram not to touch the paper with your fingers or hands? Does this matter in the food color experiment?
Post-Lab Questions 1. Why must you use a pencil, and not ink or felt-tip pen, to mark on the paper the origin of the spots of the amino acid preparations? 2. In this experiment, suppose you forgot to mark the position of the solvent front when you tested the hydrolysate of aspartame against the other amino acids. a. Is it still possible to determine how many amino acids were present in the hydrolysate? Explain your answer. b. Could you still...
11. Why should you use pencil instead of pen when marking on a thin layer chromatography plate? a. Pencil lead isn't as dark. b. The components of pen ink will separate along with your sample, while pencil lead will not. c. Pen ink will not mark on a TLC plate. d. Pen ink will undergo a chemical reaction with the solvent. 12. Which of the following is not a reason to use thin-layer chromatography? a. To determine the number of...
6. Other than a liquid solvent, what could function as the mobile phase in chromatography? 7. Why was it necessary to treat the chromatogram with DMG and ammonia? 8. Why did you use pencil to draw the bottom line instead of ink? 9. What caused the streaking of color near the staple in the chromatograms? 10. What would you observe on a chromatogram if two ions in your unknown had very similar Rf values? 6. Other than a liquid solvent,...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
1-4 1. What would happen if you used a water-soluble pen on the chromatography paper to mark your origin line? How would this affect the Re values? 2. A student accidently adds 17 ml of NaCl to their beaker rather than the 7 mL of NaCl they were suppose to add to their beaker. How might this mistake affect their chromatogram? Do you think they would be able to obtain any data? Briefly explain your answer. 10 3. Is it...
Question 5 (0.5 points) A certain compound is spotted on the starting line of a small paper chromatogram and that same compound is spotted again on a much larger paper chromatogram. Both papers are developed using the same solvent and are pulled out when the solvent is 2 cm from the top. Which statement is true regarding the R of the spot after development? C) The spot on the larger paper should have a higher Rf because the spot travels...
can you solve this for me, please Place approximately 10 mg of each of the four reference compounds in labeled vials or 10 X 75-mm test tubes and dissolve the samples in a few drops of methanol. In a fifth tube place a quarter of an analgesic tablet and add 1 mL of methanol. Crush the tablet with a rod, stir well, and allow the insoluble material to settle. Note that aspirin is not stable in methanol, so fresh solutions...
paper chromatography 2. In each part, A and B, what was the mobile phase and what was the stationary phase? CH.- -06 -COON PROCEDURE Wear protective game NOTE: Aller starting Part Aberin Parts over Dispose of all material in the con 1 the vent front to travel up the the Food Color Separation ontainer provide 1. Cut a piece of Whatman human er paper into a 14 cm draw a thin me across the wheel 2 em from one edge...