Homework Answers
4. After separating the component that is present in the food color mixture, wh have to do some other experiment to confirm the green color. After separation via any of the separating technique (column, paper, chalk, HPLC chromatographic), you have to dissolve in the solvent and you can find the green color in naked eye ad if you perform the UV vis spectroscopic study then you will get an absorbance band around 550 regions. This band reveals the green color.
5. To separate a mixture containing four amino acids, we have to perform a column chromatography. We have to increase the eluent polarity gradually to get the four different amino acids separately.
6. To separate the amino acid via paper chromatography, we have to do it very carefully. We should not touch the paper with our hand, because our body is full of amino acid which will make contamination with the paper and other amino acid mixture. During the addition of ninhydrin, it will show the different spot that is coming from our hand.
But this careness is not required for the food green color separation, because it will not be contaminated.
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paper chromatography 4. For the green food color, how would it be possible to show that the component spots you sepa...
Why did you use a pencil to mark the paper and not a pen? 1. 2....
Why did you use a pencil to mark the paper and not a pen? 1. 2. Why do you have to use a different capillary tube for each sample? Why is it very important in the amino acid chromatogram not to touch the paper with your fingers or hands? 3. 4. If two samples have identical R values does this mean that they are necessarily identical molecules? Explain What is your unknown? How do you know?
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Pre-Laboratory Questions SHOW ALL WORK TO RECEIVE CREDIT. 1. What is the purpose of the mobile...
Pre-Laboratory Questions SHOW ALL WORK TO RECEIVE CREDIT. 1. What is the purpose of the mobile phase in chromatography? 2. What is the Ry value for a component in a sample? Lab 3: Chromatography 3. A student developed chromatograms for pure substances A, B, and C, and an containing some of the substances A, B and C. Calculate the values of A, , component of the unknown mixture. Identify the substances present in the unknown pure substances A, B, and...
Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is...
Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation, you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf, which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment. Rf = Ds/Df Ds = Distance traveled by...
paper chromatography 2. In each part, A and B, what was the mobile phase and what...
paper chromatography
2. In each part, A and B, what was the mobile phase and what was the stationary phase? CH.- -06 -COON PROCEDURE Wear protective game NOTE: Aller starting Part Aberin Parts over Dispose of all material in the con 1 the vent front to travel up the the Food Color Separation ontainer provide 1. Cut a piece of Whatman human er paper into a 14 cm draw a thin me across the wheel 2 em from one edge...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
#70 & 74 Page < 3 > 0f 4 0 - leucine the-Leu. ds. 18.70 Which would you expect to be more soluble in water...
#70 & 74
Page < 3 > 0f 4 0 - leucine the-Leu. ds. 18.70 Which would you expect to be more soluble in water, a peptide rich in alanine and leucine, or a peptide rich in lysine and aspartate? Explain. 18.71 Proteins are usually less soluble in water at their isoelec- tric points. Explain familiar nctional min from alyst ? e glycyl. Applications 18.72 (a) Tyramine is said to be the "decarboxylation" product of the amino acid tyrosine. Write...
What would be my percent yield if i retrieved 3.1020 grams of soap? thank you, please show all work.
what would be my percent yield if i retrieved 3.1020 grams of
soap? thank you, please show all work.
SAPONIFICATION (HYDROLYSIS OF A FAD Fats and edible oils consitute a special class of esters called riglycerides in which alcohol part of the molecule, the trihydroxy compound glycerol, forms with three molecules of various long-chain carboxylic acids (called fatty acids). Ty reactions of simple esters can also be carried out on fats and oils,i acid-or-base-catalyzed hydrolysis. three ester linkages carboxylic acids...
Separating a Mixture, Recrystallization, pre-lab assignment could you also explain why you chose that substance for...
Separating a Mixture, Recrystallization, pre-lab
assignment
could you also explain why you chose that substance for the empty
spaces and question marks
EXPERIMENT 4 Pre-Lab Assignment Separating a Mixture, Recrystalliration Name Date 1. Complete the following flowchart which shows how to separate a mixture of sand, sodium chloride and acetanilide. Notice that after a separation process (a down arrow) the filtered solids are shown on the left and the filtrate (the liquid) is shown on the right. The terminal step...
Why did you use a pencil to mark the paper and not a pen? 1. 2. Why do you have to use a different capillary tube for each sample? Why is it very important in the amino acid chromatogram not to touch the paper with your fingers or hands? 3. 4. If two samples have identical R values does this mean that they are necessarily identical molecules? Explain What is your unknown? How do you know?
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Pre-Laboratory Questions SHOW ALL WORK TO RECEIVE CREDIT. 1. What is the purpose of the mobile phase in chromatography? 2. What is the Ry value for a component in a sample? Lab 3: Chromatography 3. A student developed chromatograms for pure substances A, B, and C, and an containing some of the substances A, B and C. Calculate the values of A, , component of the unknown mixture. Identify the substances present in the unknown pure substances A, B, and...
paper chromatography
2. In each part, A and B, what was the mobile phase and what was the stationary phase? CH.- -06 -COON PROCEDURE Wear protective game NOTE: Aller starting Part Aberin Parts over Dispose of all material in the con 1 the vent front to travel up the the Food Color Separation ontainer provide 1. Cut a piece of Whatman human er paper into a 14 cm draw a thin me across the wheel 2 em from one edge...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
#70 & 74
Page < 3 > 0f 4 0 - leucine the-Leu. ds. 18.70 Which would you expect to be more soluble in water, a peptide rich in alanine and leucine, or a peptide rich in lysine and aspartate? Explain. 18.71 Proteins are usually less soluble in water at their isoelec- tric points. Explain familiar nctional min from alyst ? e glycyl. Applications 18.72 (a) Tyramine is said to be the "decarboxylation" product of the amino acid tyrosine. Write...
what would be my percent yield if i retrieved 3.1020 grams of
soap? thank you, please show all work.
SAPONIFICATION (HYDROLYSIS OF A FAD Fats and edible oils consitute a special class of esters called riglycerides in which alcohol part of the molecule, the trihydroxy compound glycerol, forms with three molecules of various long-chain carboxylic acids (called fatty acids). Ty reactions of simple esters can also be carried out on fats and oils,i acid-or-base-catalyzed hydrolysis. three ester linkages carboxylic acids...
Separating a Mixture, Recrystallization, pre-lab
assignment
could you also explain why you chose that substance for the empty
spaces and question marks
EXPERIMENT 4 Pre-Lab Assignment Separating a Mixture, Recrystalliration Name Date 1. Complete the following flowchart which shows how to separate a mixture of sand, sodium chloride and acetanilide. Notice that after a separation process (a down arrow) the filtered solids are shown on the left and the filtrate (the liquid) is shown on the right. The terminal step...
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