Question

A microbiologist is preparing a medium on which to culture E. Colibacteria. She uses 1.0M K2HPO4 and 1.0M KH2PO4 as her conjugate acid/base pair. She buffers the medium at a pH of 7.00 to minimize the effect of acid-producing fermentation. She uses 50mL of the H2PO4-, how many mL of the HPO42- must be added. The Ka is 6.3x10-8.

Answer 1

Ans Reaction HP) H The Value CHPJ CHaP 6.3 16-8 G-3x10-S 1X16 o63 P 0632 Pou the volume volume da comL 17 CHaPaod 63 V volume .63 V d H Poy=106m 100 m d H2 POy volome - 61.341mL The 1.63 0061.3L) ml 11 volome the 38.650 m 3% 650mL V dH2 Pa