A microbiologist is preparing a medium on which to culture E.
Coli bacteria. She uses 1.5M Na2HPO4 and 1.5M KH2PO4 as her
conjugate acid/base pair. She buffers the medium at a pH of 7.00 to
minimize the effect of acid-producing fermentation. She uses 75mL
of the H2PO4-, how many mL of the HPO42- must be added. The Ka is
6.3x10-8.
A microbiologist is preparing a medium on which to culture E. Coli bacteria. She uses 1.5M...
A microbiologist is preparing a medium on which to culture E. Colibacteria. She uses 1.0M K2HPO4 and 1.0M KH2PO4 as her conjugate acid/base pair. She buffers the medium at a pH of 7.00 to minimize the effect of acid-producing fermentation. She uses 50mL of the H2PO4-, how many mL of the HPO42- must be added. The Ka is 6.3x10-8.
A microbiologist is preparing a medium on which to culture E. coli bacteria. She buffers the medium at pH 7.00 to minimize the effect of acid-producing fermentation. What volumes of equimolar aqueous solutions of K2HPO4 and KH2PO4 must she combine to make 750.0 mL of the pH 7.00 buffer? Ka values for phosphoric acid: Ka1 = 7.2 ×10−3 Ka2 = 6.3 ×10−8 Ka3 = 4.2 × 10−13 Volume H2PO4− = ??? mL Volume HPO42− = ??? mL
Cabrillo College Chemistry 1B Buffer Problem Set 1. A desires study the effect of pH n the growth of E. coli bacteria. She plans to do the research by preparing culture media that are buffered at three distinctly different PH values. Therefore, she decides to use the triprotic phosphoric acid (H,PO), potassium dihydrogen hydrogen phosphate o") and potassiam phosphate POe) to prepare the buffers K-1 7.11 103 Ka- 6.32 x 10" Kal 7.11 x 10 MATERIALSAFALLABLE Standardized solutions: 1.00 MH3PO,...