Question

questions #1-3 please

Questions. Beer's Law (1-5) The actual a value for Red 40 is 2.7 x 10 L/mole cm at 500 nm. Use this value, plus your highest absorbance reading at 500 nm, the b value of 1.0 cm and equation (1) to calculate the molarity (moles/L) of the dye. 2. The FW for Red 40 is 496 g/mole. Recalculate its concentration as g/L. Compare this value with the concentration of your original Kool Aid solution. The ratio of these two numbers (times 100) should reveal the % of Red 40 in your Kool Aid sample. A solution with a concentration of 0.14M is measured to have an absorbance of 0.43. Another solution of the same chemical is measured under the same conditions and has an 3. absorbance of 0.37. What is its concentration?

Answer 1

Absord (page-1.) (2) Acconding to Lambert - Been's Law, a orobance of a dilute homogeneous solutiad under measurement can be written as Axt.bc A - absorbance of the solutions Since the highest Esmolare extinction coefficient absorbance reading at -2.7 x 10 4 L/molem" 500nm is not giver bobath Length through which So, we say, it is a the light toroved. • please use the alasorobance (A) = 1.0cm (Given) connect numetrical value a concentration of the solution in (4) of the abran concentration in molarity. Molarity of the solution of the dye (e) > A А (2) The F of Red 40 is 496 gole. L/molean Concerforation of the solution obtained = AX 0.370370x107 A mol/L مه 2.78104 01.0cm mole/h 2,7x10x mily WAN o AX0-370370x107x4969/L). - 4x0.370370X10-4 (4) (this is the concentration of the 2(A) Solution ingle) please puse the absorbance (A) data seconded during experiment.

• % Red 40 in mentioned 4x0.370370 x 10 x496 g/L. Kool Aid Sample x 100 concentration of original Kool And solution (ingle) (3.) A solution with a concentration (1) > 0.14 (4) Heaweed absorbance (A1) >0.43 Another solution of the sampe same chemical is measured under the same conditions. It has absorbance 2 0.37 . c e (A2) Concentration of this solution (C2) 2 ? Due to the use of same chemical falland unders Same Condition, here molar extinction coefficient (E) and pathlongth must be conchanged. Az Ebe, and, A2 zebez |Eb = Al. M, Az - Az G AZA n = A2.a AL Hence the concentration of the solution having absorbance 0.37 is 0.12096 (1) 0.37 I x 0.14 (M) 0.43 1 2 = 0.12046 (4) -3 (Ans.)
As the path length is constant so the absorbance is only vary with the concentration of the solution according to Beer's law.

Answer 2

Absord (page-1.) (2) Acconding to Lambert - Been's Law, a orobance of a dilute homogeneous solutiad under measurement can be written as Axt.bc A - absorbance of the solutions Since the highest Esmolare extinction coefficient absorbance reading at -2.7 x 10 4 L/molem" 500nm is not giver bobath Length through which So, we say, it is a the light toroved. • please use the alasorobance (A) = 1.0cm (Given) connect numetrical value a concentration of the solution in (4) of the abran concentration in molarity. Molarity of the solution of the dye (e) > A А (2) The F of Red 40 is 496 gole. L/molean Concerforation of the solution obtained = AX 0.370370x107 A mol/L مه 2.78104 01.0cm mole/h 2,7x10x mily WAN o AX0-370370x107x4969/L). - 4x0.370370X10-4 (4) (this is the concentration of the 2(A) Solution ingle) please puse the absorbance (A) data seconded during experiment.

• % Red 40 in mentioned 4x0.370370 x 10 x496 g/L. Kool Aid Sample x 100 concentration of original Kool And solution (ingle) (3.) A solution with a concentration (1) > 0.14 (4) Heaweed absorbance (A1) >0.43 Another solution of the sampe same chemical is measured under the same conditions. It has absorbance 2 0.37 . c e (A2) Concentration of this solution (C2) 2 ? Due to the use of same chemical falland unders Same Condition, here molar extinction coefficient (E) and pathlongth must be conchanged. Az Ebe, and, A2 zebez |Eb = Al. M, Az - Az G AZA n = A2.a AL Hence the concentration of the solution having absorbance 0.37 is 0.12096 (1) 0.37 I x 0.14 (M) 0.43 1 2 = 0.12046 (4) -3 (Ans.)
As the path length is constant so the absorbance is only vary with the concentration of the solution according to Beer's law.